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Test Catalog

Test ID: ML1HM    
MLH1 Hypermethylation Analysis, Tumor

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, slide review will always be performed at an additional charge.

 

See Lynch Syndrome Testing Algorithm in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Varies

Advisory Information

This test is not recommended as a first-tier screening measure for hereditary nonpolyposis colon cancer (HNPCC). Refer to MSI / Microsatellite Instability (MSI), Tumor and Mismatch Repair (MMR) Protein Immunohistochemistry Only, Tumor.

 

Testing will only be performed on colon or endometrial tumors demonstrating loss of MLH1 protein expression by immunohistochemistry.

 

Mayo's preferred screening test (BRMLH / MLH1 Hypermethylation and BRAF Mutation Analysis, Tumor) includes both MLH1 promoter hypermethylation and BRAF V600E testing.

 

Extracted DNA from tissues is not an acceptable specimen type.

Necessary Information

Pathology report must accompany specimen in order for testing to be performed.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Specimen Type: Tissue block or slide

Collection Instructions:

1. Submit formalin-fixed, paraffin-embedded tissue block (preferred) or 1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides (5-micron thick sections) of the tumor tissue.

2. Sections should contain tumor tissue.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

1. Molecular Genetics: Inherited Cancer Syndromes Patient Information (T519) in Special Instructions

2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Gastroenterology and Hepatology Client Test Request (T728)

-Oncology Test Request (T729)

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis NA
Lipemia NA
Icterus NA
Other Specimens that have been decalcified (all methods); specimens that have not been formalin-fixed, paraffin-embedded; bone marrow in EDTA, extracted DNA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
VariesAmbient (preferred)
 Frozen 
 Refrigerated