TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: MSI    
Microsatellite Instability (MSI), Tumor

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, slide review will always be performed at an additional charge.

 

See Lynch Syndrome Testing Algorithm in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Varies

Shipping Instructions

Ambient specimen preferred to arrive within 96 hours of draw.

Necessary Information

Pathology report must accompany specimen in order for testing to be performed.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

This test cannot be used to assess tumor tissue unless both tumor and normal tissue are submitted.

 

If sending multiple blocks, identify individual blocks as normal or tumor.

 

Paraffin-embedded tissue blocks that have been decalcified are generally unsuccessful and not validated for testing. If a decalcified specimen is submitted (regardless of decal solution), testing will be canceled.

 

Specimen Type: Tumor tissue block, formalin-fixed, paraffin-embedded (FFPE) prepared cell block unstained slides

Specimen Volume: Approximately 1 cm(2) of tumor is required. This can be 1 cm(2) in aggregate (eg, 5 unstained slides each containing with 0.2 cm(2) of tumor and normal tissue).


Collection Instructions:

1. Submit formalin-fixed, paraffin-embedded tissue block with corresponding hematoxylin and eosin (H and E) slides (preferred) or 1 slide stained with H and E and 10 unstained, nonbaked slides (5- micrometer thick sections) of the tumor tissue.

2. Label specimen as Tumor.

 

Specimen Type: Normal tissue block or slide

Specimen Volume: Approximately 1 cm(2) of normal tissue is required. This can be 1 cm(2) in aggregate (eg, 5 unstained slides each with 0.2 cm(2) of normal tissue)

Collection Instructions:

1. Submit formalin-fixed, paraffin-embedded tissue block with corresponding hematoxylin and eosin (H and E) slides (preferred) or 1 slide stained with H and E and 10 unstained, nonbaked slides (5- micrometer thick sections) of the normal tissue.

2. Label specimen as Normal.

Additional Information:

1. Normal tissue does not have to be from the same specimen or tissue source as the tumor specimen submitted for testing. Any normal tissue block, with the exception of tissues composed primarily of adipose tissue, may be submitted. Specimens composed primarily of adipose tissue would not yield a sufficient amount of DNA and if submitted, testing will be canceled.

2. If normal tissue in a formalin-fixed, paraffin-embedded tissue block is not available, whole blood may be submitted instead (see below). A separate FFPE-tumor block is still required for testing if sending in a whole blood specimen for normal.

 

Specimen Type: Normal whole blood (if normal tissue block is not available)

Container/Tube: Lavender-top (EDTA) or yellow-top (ACD)

Acceptable: Any anticoagulant

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as Normal.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

1. Molecular Genetics: Inherited Cancer Syndromes Patient Information (T519) in Special Instructions

2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Oncology Test Request (T729)

-Gastroenterology and Hepatology Client Test Request (T728)

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Other Decalcified specimens, low tumor percentage, insufficient amount of tumor, insufficient amount of normal, adipose tissue, nonformalin fixed, fresh tissue, cytology smears

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
VariesAmbient (preferred)
 Frozen 
 Refrigerated